EBI3 Antikörper (Biotin)

Produktnummer ABIN7540543

Hersteller Produkt- Nr.: 210-306-c56

Dieses Anti-EBI3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von EBI3 in WB, ELISA, IP und Neut. Geeignet für Maus.

Kurzübersicht für EBI3 Antikörper (Biotin) (ABIN7540543)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EBI3 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Klon: V1-4F5-25

Produktdetails anti-EBI3 Antikörper (Biotin)

Hersteller Produkt- Nr.: 210-306-c56

Hersteller: Rockland

Verwendungszweck: IL-35 EBI3 Biotin Conjugated Antibody

Kreuzreaktivität (Details): This antibody is specific for mouse EBI3 protein.

Aufreinigung: This product was purified from concentrated tissue culture supernate by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Immunogen: Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.

Isotyp: IgG2b

Labeling Ratio: 10-20

Anwendungsinformationen

Applikationshinweise:

Neutralization_Dilution: 10 μg/mL

Immunoprecipitation_Dilution: 1 μg/mL

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Kommentare:

This purified antibody has been tested in Neutralization and suitable for IP. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   Restore with 0.1 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu EBI3

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Andere Bezeichnung: Ebi3

Hintergrund: Mouse anti-EBI3 biotin conjugated antibody, mouse anti-IL-35 biotin conjugated antibody, Epstein-Barr virus induced 3 protein, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35,The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.

Gen-ID: 50498

NCBI Accession: NP_056581

UniProt: O35228