EBI3 Antikörper

Produktnummer ABIN7540536

Hersteller Produkt- Nr.: dup_210-301-b66s_dup

Der Maus Monoklonal Anti-EBI3-Antikörper wurde für WB, ELISA und IP validiert. Er ist geeignet, EBI3 in Proben von Maus zu detektieren. Es sind 2+ Publikationen verfügbar.

Kurzübersicht für EBI3 Antikörper (ABIN7540536)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EBI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Klon: V1-4C4-22

Produktdetails anti-EBI3 Antikörper

Hersteller Produkt- Nr.: dup_210-301-b66s_dup

Hersteller: Rockland

Verwendungszweck: IL-35 EBI3 Antibody

Kreuzreaktivität (Details): This antibody is specific for mouse EBI3 protein.

Aufreinigung: Anti-Mouse EBI-3 (MOUSE) Monoclonal Antibody was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Sterilität: Sterile filtered

Immunogen: Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Neutralization_Dilution: 10 μg/mL

Immunoprecipitation_Dilution: 1 μg/mL

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Other: User Optimized

Kommentare:

Suggested Applications: Cellular Assay
Anti-Mouse EBI-3 antibody has been tested for use in ELISA and western Blot and is suitable for IP and Neutralization. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-EBI3 Antikörper (ABIN7540536)

Feng, Zhang, Ding, Chen, Wang, Wang, Zou, Sheng, Chen, Pang, Marshall, Shi, Nedergaard, Li, Xiao: "B lymphocytes ameliorate Alzheimer's disease-like neuropathology via interleukin-35." in: Brain, behavior, and immunity, Vol. 108, pp. 16-31, (2023) (PubMed).

Zhang, Li, Zhou, Zhang, Wu, Xu, Shen, An, Shen, Yang, Zhang, Tao: "The Unknown Aspect of BAFF: Inducing IL-35 Production by a CD5+CD1dhiFcγRIIbhi Regulatory B-Cell Subset in Lupus." in: The Journal of investigative dermatology, Vol. 137, Issue 12, pp. 2532-2543, (2017) (PubMed).

Details zu EBI3

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Andere Bezeichnung: Ebi3

Hintergrund: Mouse anti-EBI3 antibody, mouse anti-IL-35 antibody, Epstein-Barr virus induced 3 protein, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35,The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.

Gen-ID: 50498

NCBI Accession: NP_056581

UniProt: O35228