SHANK3 Antikörper (AA 586-626) (Biotin)

Produktnummer ABIN7540507

Hersteller Produkt- Nr.: 200-306-w72

Dieses Anti-SHANK3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von SHANK3 in WB, IHC und ELISA. Geeignet für Ratte.

Kurzübersicht für SHANK3 Antikörper (AA 586-626) (Biotin) (ABIN7540507)

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Reaktivität: Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SHANK3 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Klon: S367-51

Produktdetails anti-SHANK3 Antikörper (Biotin)

Bindungsspezifität: AA 586-626

Hersteller Produkt- Nr.: 200-306-w72

Hersteller: Rockland

Verwendungszweck: SHANK1/SHANK3 Antibody Biotin

Kreuzreaktivität (Details): BLAST analysis suggests that it is 100 % identical to mouse, 97 % identical to human and ~70 % identical to SHANK1 and SHANK2.

Aufreinigung: Anti-SHANK1/SHANK3 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Anti-SHANK1/SHANK3 Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 586-626 (SH3 domain) of rat SHANK3.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:10,000

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1:1000

Other: ICC/IF: 1:100

Kommentare:

Anti-SHANK1/SHANK3 Biotin Conjugated Antibody is suitable for Western Blots, Immunohistochemistry and Immunocytochemistry. Expect a band approximately ~190kDa on specific lysates or tissues. It will cross react with SHANK1, but it will not cross react with SHANK2. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.1 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu SHANK3

Target: SHANK3 (SH3 and Multiple Ankyrin Repeat Domains 3 (SHANK3))

Andere Bezeichnung: Shank3

Hintergrund: Shank postsynaptic density protein, SH3 and multiple ankyrin repeat domains 3, Proline rich synapse associated protein 2, Proline-rich synapse-associated protein 2, ProSAP2, PSAP2, , SH3 and multiple ankyrin repeat domains protein 3,SHANK proteins are scaffolding adaptors that have been shown to integrate neurotransmitter receptors into the cortical cytoskeleton at postsynaptic densities. SHANK1-3 of the SHANK/ProSAP family are molecular scaffolds in the postsynaptic density (PSD). SHANK recruits betaPIX and PAK to dendritic spines to regulate postsynaptic structure and interacts with ionotropic receptor and metabotropic glutamate receptor complexes. Transcript splice variation in the Shank family influences the spectrum of Shank-interacting proteins in the PSDs of adult and developing brain to ensure normal development. Anti-SHANK1/SHANK3 is ideal for research in Neuroscience, including autism spectrum disorder, and Cell Adhesion.

Gen-ID: 59312

UniProt: Q9JLU4

Pathways: Synaptic Membrane, Tube Formation, Regulation of long-term Neuronal Synaptic Plasticity