5-Hydroxymethylcytosine Antikörper

Produktnummer ABIN7540500

Hersteller Produkt- Nr.: 200-301-v06

Der Maus Monoklonal anti-5-Hydroxymethylcytosine Antikörper wird verwendet zum Nachweis von 5-Hydroxymethylcytosine in Proben von Human. Er wurde validiert für ELISA, DB und FM.

Kurzübersicht für 5-Hydroxymethylcytosine Antikörper (ABIN7540500)

Target: 5-Hydroxymethylcytosine (5-hmC)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser 5-Hydroxymethylcytosine Antikörper ist unkonjugiert

Applikation: ELISA, Dot Blot (DB), Fluorescence Microscopy (FM)

Produktdetails anti-5-Hydroxymethylcytosine Antikörper

Hersteller Produkt- Nr.: 200-301-v06

Hersteller: Rockland

Verwendungszweck: 5-hmc Antibody

Kreuzreaktivität (Details): Cross-reactivity with 5hmC from other sources has not been determined.

Aufreinigung: Anti-5hmC Antibody was purified by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen: Anti-5hmC Antibody was produced in mice by repeated immunization with 5-hydroxymethlcytosine.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

ELISA_Dilution: 1:500

IF_Microscopy_Dilution: User Optimized

Other: hMeDIP: 2.5 μg per IP. Dot Blot: 2 μg/mL

Kommentare:

Suggested Applications: hMeDIP: 2.5μg per IP. Dot Blot: 2 μg/ml
Anti-5hmC Antibody is tested for Dot Blotting, ELISA, and Hydroxymethylated DNA Immunoprecipitation Sequencing. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu 5-Hydroxymethylcytosine

Target: 5-Hydroxymethylcytosine (5-hmC)

Andere Bezeichnung: 5-hydroxymethylcytosine

Substanzklasse: Chemical

Hintergrund: 5-hydroxymethylcytosine,5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. So far, the 5-hmC bases have been identified in Purkinje neurons, in granule cells and embryonic stem cells where they are present at high levels (up to 0,6 % of total nucleotides in Purkinje cells). Preliminary results indicate that 5-hmC may have important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests a few putative mechanisms that could have big implications in epigenetics: 5-hydroxymethylcytosine may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine and, as such open up entirely new perspectives in epigenetic studies. Due to the structural similarity between 5-mC and 5-hmC, these bases are experimentally almost indistinguishable. Recent articles demonstrated that the most common approaches (e.g. enzymatic approaches, bisulfite sequencing) do not account for 5-hmC. The development of the affinity-based technologies appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. Anti-5hmC Antibody is ideal for research in Epigenetics and Gene Expression.