5-mC Antikörper

Produktnummer ABIN7540499

Hersteller Produkt- Nr.: 200-301-v02

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in DB, ChIP, ELISA und WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für 5-mC Antikörper (ABIN7540499)

Target: 5-mC

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Dot Blot (DB), Chromatin Immunoprecipitation (ChIP), ELISA, Western Blotting (WB)

Klon: 33D3

Produktdetails

Hersteller Produkt- Nr.: 200-301-v02

Hersteller: Rockland

Verwendungszweck: 5-mC Antibody

Kreuzreaktivität (Details): Cross-reactivity with 5-mC from other sources has not been determined.

Aufreinigung: Anti-5-mC Antibody was purified was purified by gel filtration.

Sterilität: Sterile filtered

Immunogen: Anti-5-mC Antibody was produced in mice by repeated immunization with 5-methylcytosine.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

ChIP_Dilution: 1 μg/ChIP

ELISA_Dilution: 1:3,000

Western_Blot_Dilution: 1:1,000

Other: Dot Blot: 1:250

Kommentare:

Anti-5-mC Antibody is tested for Dot Blotting, ELISA, Immunofluorescence, Methylated DNA Immunoprecipitation (MeDIP), and Sequencing (MeDIP-seq). Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: 5-mC

Hintergrund: 5-methylcytosine, 5-methyl cytosine,The 5-methylcytosine antibody (clone 33D3) is the most published and widely used antibody for DNA methylation analysis. Anti-5mC is ideal for research in Epigenetics due to its applicability to DNA methylation analysis.