5-mC Antikörper

Produktnummer ABIN7540498

Hersteller Produkt- Nr.: 200-301-u98

Dieses Anti--Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von in DB, FM, IF und IP. Geeignet für Human.

Kurzübersicht für 5-mC Antikörper (ABIN7540498)

Target: 5-mC

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Dot Blot (DB), Fluorescence Microscopy (FM), Immunofluorescence (IF), Immunoprecipitation (IP)

Produktdetails

Hersteller Produkt- Nr.: 200-301-u98

Hersteller: Rockland

Verwendungszweck: 5-mC Antibody

Kreuzreaktivität (Details): Cross-reactivity with 5-mC from other sources has not been determined.

Aufreinigung: Anti-5-mC Antibody was purified by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen: Anti-5-mC Antibody was produced in mice by repeated immunization with 5-methyl Cytidine.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: 1-2 μg/IP

IF_Microscopy_Dilution: 1:500

Other: FISH dilution: 1:200 - 1:1,000. Dot Blot: 1:600

Kommentare:

Suggested Applications: FISH
Anti-5-mC Antibody is tested for IF and Dot Blotting, Fluorescence In Situ Hybridization (FISH), Immunofluorescence and Methylated DNA Immunoprecipitation (MeDIP). Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: 5-mC

Hintergrund: 5-methylcytidine, 5-methyl Cytidine,5-methyl Cytidine is a modified nucleotide found in the DNA of both plants and vertebrates. Anti-5-mC Antibody is ideal for research in Epigenetics, specifically DNA methylation, due to its applicability to methylation analysis.