HSP70 1A Antikörper

Produktnummer ABIN7540482

Hersteller Produkt- Nr.: 200-301-f64

Der Maus Monoklonal Anti-HSP70 1A-Antikörper wurde für WB, IHC, IP und IF validiert. Er ist geeignet, HSP70 1A in Proben von Huhn zu detektieren.

Kurzübersicht für HSP70 1A Antikörper (ABIN7540482)

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Reaktivität: Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSP70 1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Klon: BB70

Produktdetails anti-HSP70 1A Antikörper

Hersteller Produkt- Nr.: 200-301-f64

Hersteller: Rockland

Verwendungszweck: Hsp70/Hsc70 Antibody

Kreuzreaktivität (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp70/Hsc70 from Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast sources based on 100 % homology with the immunizing sequence.

Aufreinigung: Anti-Hsp70/Hsc70 Antibody was purified by Protein G chromatography.

Sterilität: Sterile filtered

Immunogen: Hsp70/Hsc70 Antibody was produced in mice by repeated immunizations raised against chicken Hsp70/Hsp90 complex.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1 μg/mL

Kommentare:

Anti-Hsp70/Hsc70 Antibody is tested for WB, IP, IF, and IHC. Expect a band approximately ~72 and ~73kDa proteins corresponding to the molecular mass of inducible Hsp and Hsc on SDS PAGE immunoblots. This monoclonal antibody reacts with chicken Hsp70/Hsp90 complex. Specific conditions for reactivity should be optimized by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu HSP70 1A

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Andere Bezeichnung: HSPA1A

Hintergrund: Hsp70 1, Hsp70 2, Hsp70.1, Hsp72, Hsp73, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2,Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Gen-ID: 3303

NCBI Accession: NP_005336

UniProt: P0DMV8

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process