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KDELR1 Antikörper (C-Term)

Dieses Anti-KDELR1-Antikörper ist ein Mouse Monoclonal-Antikörper zur Detektion von KDELR1 in WB, IF, FM. Geeignet für Saccharomyces cerevisiae.
Rockland
Produktnummer ABIN7540480
Hersteller Produkt- Nr.: 200-301-f44

Kurzübersicht für KDELR1 Antikörper (C-Term) (ABIN7540480)

Target

Alle KDELR1 Antikörper anzeigen
KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))

Reaktivität

  • 17
  • 5
  • 5
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Saccharomyces cerevisiae

Wirt

  • 16
  • 3
Maus

Klonalität

  • 14
  • 5
Monoklonal

Konjugat

  • 14
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser KDELR1 Antikörper ist unkonjugiert

Applikation

  • 19
  • 8
  • 6
  • 5
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Klon

2E7
  • Bindungsspezifität

    • 9
    • 4
    • 2
    • 1
    • 1
    C-Term

    Hersteller Produkt- Nr.

    200-301-f44

    Hersteller

    Rockland

    Verwendungszweck

    HDEL Antibody

    Kreuzreaktivität (Details)

    A BLAST analysis was used to suggest cross-reactivity with HDEL from Drosophilia, yeast, Saccharomyce, and Plants (Barnyard grass, beet, cotton, mung bean, sorghum, wheat) based on 100 % homology with the immunizing sequence.

    Aufreinigung

    Anti-HDEL Antibody was purified by Protein G chromatography.

    Sterilität

    Sterile filtered

    Immunogen

    HDEL Antibody was produced in mice by repeated immunizations raised against a synthetic HDEL synthetic peptide corresponding to the C-terminus of yeast Bip.

    Isotyp

    IgG2b
  • Applikationshinweise

    IF_Microscopy_Dilution: 1:50-1:500

    Western_Blot_Dilution: 1:1000-1:2000

    Kommentare

    Anti-HDEL Antibody is tested for use in WB and IF microscopy. Expect a band approximately ~78kDa corresponding to specific lysates. Specific conditions for reactivity should be optimized by the end user.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: 0.09 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Target

    KDELR1 (KDEL (Lys-Asp-Glu-Leu) Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1))

    Andere Bezeichnung

    ERD2

    Hintergrund

    H-D-E-L, endoplasmic reticulum, luminal ER protein retention,HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat.

    Pathways

    Maintenance of Protein Location
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