Rekombinanter CD8 Antikörper (Extracellular Domain) (Atto 647N)

Produktnummer ABIN7540335

Dieses Alpaca Monoklonal-Antikörper erkennt spezifisch CD8 in FACS, IF, IF (p) und IF/ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter CD8 Antikörper (Extracellular Domain) (Atto 647N) (ABIN7540335)

Target: CD8

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Human

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Dieser CD8 Antikörper ist konjugiert mit Atto 647N

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (fixed cells) (IF/ICC)

Klon: 3ALB177

Produktdetails anti-CD8 Antikörper (Atto 647N)

Bindungsspezifität: Extracellular Domain

Expressionssystem: E.coli

Verwendungszweck: Anti-CD8 (Human) LIMAAbody® is a carefully developed and validated polyclonal nanobody (single-domain antibody) binding to human CD8 highly suitable for tissue stainings.

Marke: LIMAAbody®

Spezifität: Detects endogenous levels of total human CD8 alpha protein.

Kreuzreaktivität (Details): not analysed

Produktmerkmale: VHH protein tag: C-terminal (6x) His-Tag

Aufreinigung: Affinity-purified antibody fragment.

Immunogen: Recombinant protein containing the extracellular domain of human CD8 protein.

Anwendungsinformationen

Applikationshinweise: Immunofluorescence: 10 μg/mL (frozen sections), 10 μg/mL (cell culture), 20 μg/mL (wholemount), 10 μg/mL (FACS)

Kommentare:

VHH protein tag: C-terminal (6x) His-Tag

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.4, 0.02% sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Upon receipt store at 4°C. Stable for 6 months. Do not freeze.

Haltbarkeit: 6 months

Details zu CD8

Target: CD8

Andere Bezeichnung: CD8

Hintergrund: CD8, known as Cluster of Differentiation 8, forms a disulphide-linked heterodimer comprising unrelated α and β subunits. Each subunit, characterized as a glycoprotein, is structured with a single extracellular Ig-like domain, a polypeptide linker, a transmembrane component, and a concise cytoplasmic tail. Within T cells, CD8 serves as the co-receptor alongside the T cell receptor (TCR). These distinct structures collaboratively identify the Antigen-Major Histocompatibility Complex (MHC). Specifically, the interaction occurs between the Ig-like domain of CD8α and the α3-domain of the MHC class I molecule. CD8 plays a pivotal role in ensuring the specificity of the TCR-antigen interaction, extending the duration of contact between the T cell and the antigen-presenting cell. Moreover, the α chain of CD8 recruits the tyrosine kinase Lck, an indispensable component for initiating T cell activation .

Molekulargewicht: 26 kDa

NCBI Accession: NP_001759

UniProt: P01732