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mCherry Antikörper

Name: mCherry Antikörper
Brand: antibodies-online
SKU: ABIN7505996
Price: 463.75 EUR
Availability: InStock

Dieses Anti-mCherry-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von mCherry in WB, ICC und FACS. Geeignet für Anaplasma marginale.

Western blotting analysis of mCherry fluorescent protein using rabbit polyclonal PAb (918) on lysates of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs (HEK293T/17 cells transfected with YFP/GPI, negative control) under reducing and non-reducing conditions. (mCherry Antikörper)

Flow cytometry surface staining pattern of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained using anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC). (mCherry Antikörper)

FACS

Separation of HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs stained anti-mCherry Purified rabbit polyclonal antibody (concentration in sample 2 μg/mL, GAR APC, red-filled) from HEK293T/17 cells co-transfected with mCherry/GPI and YFP/GPI constructs unstained by primary polyclonal antibody (GAR APC, black-dashed) in flow cytometry analysis (surface staining) of HEK293T17/mCherry/YFP cell suspension. (mCherry Antikörper)

FACS

3 Abbildungen

Produktnummer ABIN7505996

Kurzübersicht für mCherry Antikörper (ABIN7505996)

Target

mCherry (mCherry Fluorescent Protein)

Reaktivität

Anaplasma marginale

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser mCherry Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Verwendungszweck

Anti-mCherry purified

Spezifität

The rabbit polyclonal antibody PAb (918) recognizes a red fluorescent protein tag mCherry.

Aufreinigung

Purified by ligand affinity chromatography.

Immunogen

mCherry protein from Anaplasma marginale
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Applikationshinweise

Flow cytometry: Recommended dilution: 1-4 μg/mL, extracellular staining or intracellular staining - depending on expression.
Western blotting: Recommended dilution: 1-2 μg/mL.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Konzentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C

Informationen zur Lagerung

Store at 2-8°C. Do not freeze.
Target

mCherry (mCherry Fluorescent Protein)

Andere Bezeichnung

mCherry

Hintergrund

The mCherry is a red fluorescent protein with excitation maximum 587 nm and emission maximum 610 nm. It has around 28 kDa, and it is being used as a fluorescent tag in expression systems.

UniProt

X5DSL3