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Rekombinanter T Cell Receptor Antikörper

Reaktivität: Maus FACS, IP, Crys, IHC Wirt: Kaninchen Chimeric H57-597 unconjugated Recombinant Antibody
Produktnummer ABIN7487760
  • Target
    T Cell Receptor
    Antikörpertyp
    Recombinant Antibody
    Reaktivität
    • 6
    • 5
    Maus
    Wirt
    • 4
    • 3
    • 2
    • 2
    Kaninchen
    Klonalität
    • 9
    • 2
    Chimeric
    Konjugat
    • 10
    • 1
    Unkonjugiert
    Applikation
    • 11
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Crystallization (Crys), Immunohistochemistry (IHC)
    Verwendungszweck
    Anti-TCR [H57-597], Rabbit IgG, lambda
    Spezifität
    MAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
    Produktmerkmale

    Original Species of Ab: Hamster, Armenian

    Original Format of Ab: IgG

    Aufreinigung
    Protein A affinity purified
    Immunogen
    Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
    Klon
    H57-597
    Isotyp
    IgG lambda
  • Applikationshinweise
    In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2. H57-597 has also been used to construct a Fab fragment which has been complexed with deglycosylated TCRs for their crystallisation for structure determination (Liu 1996). Flow cytometry is another application of this antibody, where H57-597 has been used to analyse TCR-beta expression in different T cell populations (Tsukumo 2006).
    Kommentare

    This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    PBS with 0.02 % Proclin 300.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target
    T Cell Receptor
    Andere Bezeichnung
    TCR
    Hintergrund
    TCR-beta, T cell receptor, T-cell receptor, T cell receptor beta chain, TCRB, TRB, TCB1, Ti
    UniProt
    P01852
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