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Rekombinanter T Cell Receptor Antikörper

Reaktivität: Maus FACS, IP, Crys, IHC Wirt: Hamster Monoclonal H57-597 unconjugated Recombinant Antibody
Produktnummer ABIN7487759
  • Target
    T Cell Receptor
    Antikörpertyp
    Recombinant Antibody
    Reaktivität
    • 6
    • 5
    Maus
    Wirt
    • 4
    • 4
    • 2
    • 1
    Hamster
    Klonalität
    • 8
    • 3
    Monoklonal
    Konjugat
    • 10
    • 1
    Unkonjugiert
    Applikation
    • 11
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Crystallization (Crys), Immunohistochemistry (IHC)
    Verwendungszweck
    Anti-TCR [H57-597], Hamster (Armenian) IgG, lambda
    Spezifität
    MAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
    Produktmerkmale

    Original Species of Ab: Hamster, Armenian

    Original Format of Ab: IgG

    Aufreinigung
    Protein A affinity purified
    Immunogen
    Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
    Klon
    H57-597
    Isotyp
    IgG lambda
  • Applikationshinweise
    In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2. H57-597 has also been used to construct a Fab fragment which has been complexed with deglycosylated TCRs for their crystallisation for structure determination (Liu 1996). Flow cytometry is another application of this antibody, where H57-597 has been used to analyse TCR-beta expression in different T cell populations (Tsukumo 2006).
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    PBS with 0.02 % Proclin 300.
    Konservierungsmittel
    ProClin
    Vorsichtsmaßnahmen
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target
    T Cell Receptor
    Andere Bezeichnung
    TCR
    Hintergrund
    TCR-beta, T cell receptor, T-cell receptor, T cell receptor beta chain, TCRB, TRB, TCB1, Ti
    UniProt
    P01852
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