Rekombinanter PROC Antikörper

Produktnummer ABIN7487754

Der Kaninchen Chimeric Anti-PROC-Antikörper wurde für ELISA, Purif und BP validiert. Er ist geeignet, PROC in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter PROC Antikörper (ABIN7487754)

Target: PROC (Vitamin K-dependent protein C (PROC))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser PROC Antikörper ist unkonjugiert

Applikation: ELISA, Purification (Purif), Blocking Peptide (BP)

Klon: HPC-4

Produktdetails anti-PROC Antikörper

Verwendungszweck: Anti-Protein C [HPC-4], Rabbit IgG, kappa

Spezifität: Specifically binds to a specific twelve peptide sequence (EDQVDPRLIDGK) in the activation region of the Protein C (spanning the thrombin cleavage site). Does not react with activated protein C. Has been shown to be cross-reactive with baboon Protein C but not with bovine or mouse Protein C.

Kreuzreaktivität: Pavian

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: This antibody was raised by immunising mice with human protein C.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: HPC-4 binds human protein C only in the presence of Ca²⁺, it binds to the reduced carboxymethylated protein C heavy chain, and it blocks protein C activation but does not bind or inactivate protein C or activated protein C bound to its plasma inhibitor. Furthermore, it is an anti-epitope tag antibody, as it recognizes the EDQVDPRLIDGK sequence (Taylor Jr et al., 1987, 3102560). It has been shown to work in ELISAs to determine expression levels (Velander et al., 1992, PMID: 1465430). HPC-4 has been shown to be effective at purifying proteins tagged with its epitope (Rezaie et al., 1992, PMID: 1283093). It has also been used to block the activation of Protein C by the thrombin-thrombomodulin complex (Stearns et al., 1988, PMID: 2447082) and was used for the same purpose in Baboons to lower the threshold of sepsis (Taylor Jr et al., 1987, PMID: 3102560). HPC-4 has been used in many other studies to observe the function of Protein C by knocking out its function in a Ca²⁺-dependent manner, purification, and others (Tanaka et al., 2006, PMID: 16398653) (Bae et al., 2007, PMID: 17823308).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu PROC

Target: PROC (Vitamin K-dependent protein C (PROC))

Andere Bezeichnung: Protein C

Hintergrund: Autoprothrombin IIA, C-tag, Protein C tag, Protein C-tag, HPC4, Vitamin K-dependent protein C, Anticoagulant protein C, Blood coagulation factor XIV, PROC

UniProt: P04070