Rekombinanter CD28 Antikörper

Produktnummer ABIN7487692

Dieses Anti-CD28-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CD28 in FACS und ELISA. Geeignet für Human.

Kurzübersicht für Rekombinanter CD28 Antikörper (ABIN7487692)

Target: CD28

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD28 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA

Klon: 15E8

Produktdetails anti-CD28 Antikörper

Verwendungszweck: Anti-CD28 [15E8], Rabbit IgG, kappa

Spezifität: This antibody binds human CD28, which is found on a sub-population of T cells and activated B cells. The antibody does not react with B cells, granulocytes or monocytes. CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original mouse antibody was generated by immunizing mice with human T lymphocytes.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: This antibody works independently of TcR/CD3 and activation of protein kinase C (PKC) for activation of T cells and nehances cytokine secretion (PMID: 1647959). This antibody is capable of stimulating T cells in combination with either murine OKT3 antibody or CDR-mutated OKT3 Fab variants. It was found that cross-linking of specific tumor antigens with the T-cell-associated CD3 and CD28 antigens can increase IL-2 secretion, proliferation and antigen-specific cytotoxicity in resting T cells. This cross-linking can be achieved effectively by bispecific monoclonal antibodies (BiMAb) with specificity for both the tumor antigen and CD3 or CD28 antigen. A combination of bispecific antibodies BiMAb OKT3/HRS-3 with reactivity against both CD3 and Hodgkin's-lymphoma-associated CD30 antigen and BiMAb 15E8/HRS-3 with reactivity to both CD28 and CD30 antigen was able to activate resting T cells, represented by Jurkat cells (CD3+/CD28+) to produce IL-2 (PMID: 7686889). This antibody was also used in another study wherein the combined use of two CD3 x CD19 plus CD28 x CD22 bispecific antibodies induced optimal interleukin 2 secretion by Jurkat T-cell acute lymphocytic leukemia cells in the presence of target B-cells (PMID: 7689932). This antibody also provides a co-stimulatory signal to T cells. The scFv fragment of this antibody induced proliferation of peripheral blood mononuclear cells in the presence of anti-CD3 or anti-CD2 mAb and enhanced anti-tumor activity of anti-MUC1xanti-CD3 bispecific antibody when tested with lymphokine-activated killer cells with T cell phenotype (PMID: 10913626). The humanzied version of this antibody is available on request.

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD28

Target: CD28

Andere Bezeichnung: CD28

Hintergrund: T-cell-specific surface glycoprotein CD28, TP44

UniProt: P10747

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response