Rekombinanter CD163 Antikörper

Produktnummer ABIN7487686

Dieses Anti-CD163-Antikörper ist ein Kaninchen Chimeric-Antikörper zur Detektion von CD163 in FACS, ELISA und IF. Geeignet für Schwein.

Kurzübersicht für Rekombinanter CD163 Antikörper (ABIN7487686)

Target: CD163

Antikörpertyp: Recombinant Antibody

Reaktivität: Schwein

Wirt: Kaninchen

Klonalität: Chimeric

Konjugat: Dieser CD163 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

Klon: 2A10

Produktdetails anti-CD163 Antikörper

Verwendungszweck: Anti-CD163 [2A10 (2A10/11)], Rabbit IgG, lambda

Spezifität: This antibody recognizes and binds porcine CD163.

Produktmerkmale:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Aufreinigung: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages.

Isotyp: IgG lambda

Anwendungsinformationen

Applikationshinweise: This antibody is recommended to be used as a tool in the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions. This antibody can recognize porcine CD163 in a western blot assay and an immunoprecipitation assay (PMID: 9151393). 2A10 successfully binds PAM cells in a flow cytometric analysis (PMID:23785195). Porcine CD163 has also been analyzed as potential antigen targeting receptors using mouse IgG as surrogate antigens. MAbs to CD163 induced in vitro PBMC proliferation at concentrations 50-80 fold lower than the control mAb when using, as responder cells, cells from pigs immunized with mouse serum IgGs (PMID:21557980). It is also reported that porcine mAb 2A10 antigen is homologous to human CD163 and related to macrophage differentiation (PMID: 10227997). This antibody was also used to study the role of CD163 in the process of infection of porcine monocytes/macrophages by African swine fever virus infection (PMID: 14648288). This mAb was used in a sandwich enzyme-linked immunoassay (ELISA) to measure levels of sCD163 in porcine sera and biological fluids (Pérez et al, 2008).

Kommentare:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Details zu CD163

Target: CD163

Andere Bezeichnung: CD163

Hintergrund: M130, sCD163, Scavenger receptor cysteine-rich type 1 protein M130

UniProt: Q2VL90