Fibronectin Antikörper

Produktnummer ABIN7478856

Dieses Anti-Fibronectin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Fibronectin in IF, ELISA, IHC (p), IHC (fro) und RIA. Geeignet für Maus.

Kurzübersicht für Fibronectin Antikörper (ABIN7478856)

Target: Fibronectin

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Fibronectin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Produktdetails anti-Fibronectin Antikörper

Spezifität: Mouse fibronectin

Kreuzreaktivität (Details): Mouse

Produktmerkmale: This antibody may be used to identify mouse fibronectin in experimental studies on wound healing, tumour progression, and tumour invasion, etc. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.

Aufreinigung: Protein G affinity purification

Immunogen: Purified fibronectin from murine plasma

Anwendungsinformationen

Applikationshinweise: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Mouse skin,IFA, Immunohistochemistry on frozen and paraffin sections, ELISA, RIA,IHC 60 min at RT or 2-8 °C over night,IHC (frozen): 1:1000, ELISA:1:20000 (OD 0.5) Optimal working dilutions should, however, be determined by antibody titration.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 5 mg/mL

Buffer: Affinity purified (ProtG) antiserum lyophilized from phosphate buffered solution (non-sterile). Contains no BSA or sodium azide! Reconstitute with 0.5 mL of sterile water and store aliquots at -20 °C. For further dilution use appropriate antibody diluent, e.g. Art. No. PU002 for IHC.

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Details zu Fibronectin

Target: Fibronectin