Dieses Anti-Vaccinia Virus-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Vaccinia Virus in IHC, WB und EIA. Geeignet für Vaccinia Virus (VACV).
Kurzübersicht für Vaccinia Virus Antikörper (Peroxidase (POD)) (ABIN7478353)
Target
Vaccinia Virus (VACV)
(Vaccina Virus (VACV))
Reaktivität
Vaccinia Virus (VACV)
Wirt
Kaninchen
Klonalität
Polyklonal
Konjugat
Dieser Vaccinia Virus Antikörper ist konjugiert mit Peroxidase (POD)
Applikation
Immunohistochemistry (IHC), Western Blotting (WB), Enzyme Immunoassay (EIA)
Spezifität
Purified virions
Kreuzreaktivität (Details)
Does not crossreact with Parainfluenza (1-3), RSV, Adeno, Influenza A&B or HSV1, This antibody is known to react with the B5R and the A33R proteins of the virus. UNINFECTED CELL REACTIVITY: Does not react with uninfected cells. Reactive with the Lister & MVA strains of Vaccinia and Monkeypox
Aufreinigung
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
TITER : >1:1,000 by indirect ELISA. Functions in IFA, IHC & PRNT, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Konservierungsmittel
Thimerosal (Merthiolate)
Vorsichtsmaßnahmen
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.