Der Kaninchen Polyklonal Anti-Vaccinia Virus-Antikörper wurde für Func validiert. Er ist geeignet, Vaccinia Virus in Proben von Vaccinia Virus (VACV) zu detektieren.
Kurzübersicht für Vaccinia Virus Antikörper (FITC) (ABIN7478352)
Target
Vaccinia Virus (VACV)
(Vaccina Virus (VACV))
Reaktivität
Vaccinia Virus (VACV)
Wirt
Kaninchen
Klonalität
Polyklonal
Konjugat
Dieser Vaccinia Virus Antikörper ist konjugiert mit FITC
Applikation
Functional Studies (Func)
Spezifität
Purified virions
Kreuzreaktivität (Details)
Does not crossreact with Parainfluenza (1-3), RSV, Adeno, Influenza A&B or HSV1, This antibody is known to react with the B5R and the A33R proteins of the virus. UNINFECTED CELL REACTIVITY: Does not react with uninfected cells. Reactive with the Lister & MVA strains of Vaccinia and Monkeypox
Aufreinigung
This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
TITER : >1:1,000 by indirect ELISA. Functions in IFA, IHC & PRNT, Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
Lot specific
Buffer
The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.