Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch M. Tuberculosis in IHC, WB und EIA. Es zeigt Reaktivität gegenüber Proben von Mycobacterium tuberculosis.
Kurzübersicht für M. Tuberculosis Antikörper (Peroxidase (POD)) (ABIN7478288)
Target
M. Tuberculosis
Reaktivität
Mycobacterium tuberculosis
Wirt
Kaninchen
Klonalität
Polyklonal
Konjugat
Peroxidase (POD)
Applikation
Immunohistochemistry (IHC), Western Blotting (WB), Enzyme Immunoassay (EIA)
Spezifität
Antibody is reactive with numerous antigens
Kreuzreaktivität (Details)
This antiserum has not been absorbed & may react with related microorganisms. Antibody is reactive with other Mycobacteria species including: M. avium, M. phlei, and M. parafortuitum. Antibody is non-reactive with E. coli K12, Salmonella typhimurium, Pseudomonas aeruginosa, Streptococcus (group B), Candida albicans and Neisseria meningitidis. Others no tested. Antibody is reactive with the following antigens of M. tuberculosis: Lipoarabinomannan, ESAT 6, CFP10, 38 kDa, Antigen 16 (HspX), Hsp 65 (GroEl) and MoeX. Others not tested.
Aufreinigung
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
Reaktivität: Mycobacterium tuberculosis
IHC, IFA
Wirt: Kaninchen
Polyclonal
Biotin
Applikationshinweise
TITER : > 1:1,000 by ELISA, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Konservierungsmittel
Thimerosal (Merthiolate)
Vorsichtsmaßnahmen
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.