Kurzübersicht für Canine Heartworm Antikörper (Peroxidase (POD)) (ABIN7478264)
Target
Canine Heartworm
(Heartworm, Canine)
Reaktivität
Dirofilaria immitis
Wirt
Ziege
Klonalität
Polyklonal
Konjugat
Dieser Canine Heartworm Antikörper ist konjugiert mit Peroxidase (POD)
Applikation
Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (IHC)
Spezifität
Soluble & structural antigens of the adult worm
Kreuzreaktivität (Details)
Antiserum is not absorbed for related filarial Nematodes UNINFECTED CELL REACTIVITY: Non-reactive with canine serum
Aufreinigung
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
TITER : > 1:6,000 by ELISA, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Konservierungsmittel
Thimerosal (Merthiolate)
Vorsichtsmaßnahmen
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.