E. Coli Antikörper (Peroxidase (POD))

Produktnummer ABIN7478178

Produktdetails anti-E. Coli Antikörper (Peroxidase (POD))

Target: E. Coli

Reaktivität: E. coli

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser E. Coli Antikörper ist konjugiert mit Peroxidase (POD)

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (IHC)

Spezifität: Many "O" and "K" antigenic serotypes of Escherichia coli

Kreuzreaktivität (Details): Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae

Aufreinigung: This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

Immunogen: Mixture of E. coli serotypes

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.

Details zu E. Coli

Target: E. Coli

Abstract: E. Coli Produkte

Substanzklasse: Bacteria