E. Coli Antikörper (Peroxidase (POD))

Produktnummer ABIN7478174

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch E. Coli in EIA, WB und IHC. Es zeigt Reaktivität gegenüber Proben von E. coli.

Kurzübersicht für E. Coli Antikörper (Peroxidase (POD)) (ABIN7478174)

Target: E. Coli

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser E. Coli Antikörper ist konjugiert mit Peroxidase (POD)

Applikation: Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-E. Coli Antikörper (Peroxidase (POD))

Spezifität: All "O" and "K" antigenic serotypes of Escherichia coli

Kreuzreaktivität (Details): Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae

Aufreinigung: This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

Immunogen: Mixtures of all antigenic serotypes

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: TITER : > 1:2,000 by ELISA vs serotypes O157:H7, O20, O125, O55, O111 & K12
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays. Will remove E. coli proteins from recombinant preparations

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.

Details zu E. Coli

Target: E. Coli

Substanzklasse: Bacteria