Borrelia Burgdorferi Antikörper (Peroxidase (POD))
Kurzübersicht für Borrelia Burgdorferi Antikörper (Peroxidase (POD)) (ABIN7478142)
Target
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
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Spezifität
- Reactive Western Blot bands at 83kD, 41kD, 34kD and 31kD + additional low MW bands
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Kreuzreaktivität (Details)
- Cross-reacts with Treponema pallidum, B. hermsii & B. parkerii
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Aufreinigung
- This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
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Immunogen
- Whole cell preparation from B. burgdorferi
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Isotyp
- IgG
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Applikationshinweise
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TITER : >1:1,000 in Western Blot. >1:40,000 in avidin-biotin IFA
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays, Functions well in formalin fixed/paraffin embedded settings -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- Lot specific
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Buffer
- This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
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Konservierungsmittel
- Thimerosal (Merthiolate)
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Vorsichtsmaßnahmen
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
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- Borrelia Burgdorferi
Target
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