Rekombinanter VWF Antikörper (AA 1815-1939)
Kurzübersicht für Rekombinanter VWF Antikörper (AA 1815-1939) (ABIN7477621)
Target
Alle VWF Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
-
-
Bindungsspezifität
- AA 1815-1939
-
Immunogen
- Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)
-
Isotyp
- IgG
-
-
-
-
Applikationshinweise
- IHC 1:50-1:100
-
Kommentare
-
Positive Control: Colon: A moderate to strong endothelial vWF immunostaining should be seen in a fraction of blood vessels.
Negative Control: Colon: vWF immunostaining should be completely absent in epithelial and muscular cells.
-
Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Lagerung
- 4 °C,-20 °C,-80 °C
-
Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- VWF (Von Willebrand Factor (VWF))
-
Andere Bezeichnung
- von Willebrand Factor / vWF
-
Hintergrund
- Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2,von Willebrand Disease (vWD),von Willebrand Factor / vWF antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P04275
Target
-