Rekombinanter Vimentin Antikörper (AA 2-466)
Kurzübersicht für Rekombinanter Vimentin Antikörper (AA 2-466) (ABIN7477619)
Target
Alle Vimentin (VIM) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 2-466
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Immunogen
- A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:100-1:200
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Kommentare
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Positive Control: Liver: Kupffer cells must show a strong staining and sinusoidal endothelial cells should show an at least a weak staining. Colon: Endothelial and muscle cells of large vessels and stromal cells must show a strong Vimentin staining while dispersed intraepithelial T-cells must show an at least moderate intensity staining.
Negative Control: Liver: Hepatocytes cells must be Vimentin negative. Colon: All epithelial cells must be negative.
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Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Vimentin (VIM)
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Andere Bezeichnung
- Vimentin
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Hintergrund
- VIM,Vimentin antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P08670
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Pathways
- Caspase Kaskade in der Apoptose
Target
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