Rekombinanter SOX9 Antikörper (AA 393-508)

Produktnummer ABIN7477587

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch SOX9 in IHC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Rekombinanter SOX9 Antikörper (AA 393-508) (ABIN7477587)

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser SOX9 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-709R

Produktdetails anti-SOX9 Antikörper

Bindungsspezifität: AA 393-508

Immunogen: Recombinant humanSOX9 protein fragment (around aa 393- 508) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Colon: A strong SOX9 immunostaining should be seen in epithelial cells of the crypt base.

Negative Control: Colon: SOX9 immunostaining should be absent in all non-epithelial cell types (except few cells in lymphoid germinal centres - if present).

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu SOX9

Target: SOX9 (SRY (Sex Determining Region Y)-Box 9 (SOX9))

Andere Bezeichnung: SOX9

Hintergrund: Campomelic Dysplasia Autosomal Sex Reversal (CMD1), SRA1, SRXX2, SRY (sex determining region Y) box 9, SRY related HMG box gene 9, Transcription factor SOX9,SOX9 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P48436

Pathways: EGFR Signaling Pathway, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development