Rekombinanter Synaptophysin Antikörper (AA 274-313)
Kurzübersicht für Rekombinanter Synaptophysin Antikörper (AA 274-313) (ABIN7470669)
Target
Alle Synaptophysin (SYP) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 274-313
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Verwendungszweck
- Synaptophysin antibody (462R)
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Immunogen
- Recombinant fragment (around aa 274-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:100-1:200
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Kommentare
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Positive Control: Colon: A moderate to strong staining of the axons of the Auerbach's and Meissner's plexus should be seen while the endocrine cells of the mucosa and the peripheral nerves in the mucosa must show an at least a weak to moderate staining. Pancreas: A strong staining should be seen in islets of Langerhans and in peripheral nerve fibres located between the exocrine cells.
Product Description: Synaptophysin antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: All non-endocrine epithelial cells and lymphocytes should not show any staining.
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Protokoll
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
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Haltbarkeit
- 24 months
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- Synaptophysin (SYP)
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Andere Bezeichnung
- Synaptophysin
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Hintergrund
- Gene Alias: Major synaptic vesicle protein p38, MRX96, MRXSYP, Syn p38, Syp, SYPH
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UniProt
- P08247
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Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity
Target
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