Rekombinanter Synaptophysin Antikörper (AA 274-313)

Produktnummer ABIN7477577

Der Kaninchen Monoklonal Anti-Synaptophysin-Antikörper wurde für IHC validiert. Er ist geeignet, Synaptophysin in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Synaptophysin Antikörper (AA 274-313) (ABIN7477577)

Target: Synaptophysin (SYP)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Synaptophysin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-462R

Produktdetails anti-Synaptophysin Antikörper

Bindungsspezifität: AA 274-313

Immunogen: Recombinant fragment (around aa 274-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Colon: A moderate to strong staining of the axons of the Auerbach's and Meissner's plexus should be seen while the endocrine cells of the mucosa and the peripheral nerves in the mucosa must show an at least a weak to moderate staining. Pancreas: A strong staining should be seen in islets of Langerhans and in peripheral nerve fibres located between the exocrine cells.

Negative Control: Colon: All non-endocrine epithelial cells and lymphocytes should not show any staining.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu Synaptophysin

Target: Synaptophysin (SYP)

Andere Bezeichnung: Synaptophysin

Hintergrund: Major synaptic vesicle protein p38, MRX96, MRXSYP, Syn p38, Syp, SYPH,Synaptophysin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P08247

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity