Occludin Antikörper (AA 282-415)

Produktnummer ABIN7477543

This anti-Occludin antibody (ABIN7477543) is a Mouse Monoclonal antibody detecting Occludin in IHC. Suitable for Human.

Quick Overview for Occludin Antikörper (AA 282-415) (ABIN7477543)

Target: Occludin (OCLN)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Occludin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-415M

Produktdetails anti-Occludin Antikörper

Bindungsspezifität: AA 282-415

Immunogen: ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.

Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu Occludin

Target: Occludin (OCLN)

Andere Bezeichnung: Occludin

Hintergrund: BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin,Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C