Occludin Antikörper (AA 282-415)
Kurzübersicht für Occludin Antikörper (AA 282-415) (ABIN7470652)
Target
Alle Occludin (OCLN) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
-
-
Bindungsspezifität
- AA 282-415
-
Verwendungszweck
- Occludin antibody (415M)
-
Immunogen
- ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)
-
Isotyp
- IgG1, kappa
-
-
-
-
Applikationshinweise
- IHC 1:100-1:200
-
Kommentare
-
Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.
Product Description: Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.
-
Protokoll
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Lagerung
- 4 °C,-20 °C,-80 °C
-
Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
-
Haltbarkeit
- 24 months
-
-
- Occludin (OCLN)
-
Andere Bezeichnung
- Occludin
-
Hintergrund
- Gene Alias: BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin
-
UniProt
- Q16625
-
Pathways
- Cell-Cell Junction Organization, Hepatitis C
Target
-