Rekombinanter Elastin Antikörper
Kurzübersicht für Rekombinanter Elastin Antikörper (ABIN7477467)
Target
Alle Elastin (ELN) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Immunogen
- Recombinant full-length human Elastin protein
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:100-1:200
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Kommentare
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Positive Control: Colon: A moderate to strong elastin immunostaining should be seen of fibres in the lamina propria, around smooth muscle cells of the muscularis mucosae, and in blood vessels (subendothelial).
Negative Control: Colon: Elastin immunostaining should be absent in all epithelial cells.
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Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Elastin (ELN)
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Andere Bezeichnung
- Elastin
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Hintergrund
- Elastin, ELN, SVAS, Tropoelastin, WBS, WS,Elastin antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P15502
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Pathways
- Regulation of Actin Filament Polymerization
Target
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