Rekombinanter KRT14 Antikörper (AA 350-472)
Kurzübersicht für Rekombinanter KRT14 Antikörper (AA 350-472) (ABIN7477443)
Target
Alle KRT14 Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- AA 350-472
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Immunogen
- Recombinant human KRT14 fragment (around aa350-472) (exact sequence is proprietary)
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Isotyp
- IgG
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Applikationshinweise
- IHC 1:100-1:200
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Kommentare
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Positive Control: Tonsil: A strong KRT14 immunostaining should be seen in the surface and crypt epithelium.
Negative Control: Tonsil: KRT14 immunostaining should be absent in all non-epithelial cells.
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Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- KRT14 (Keratin 14 (KRT14))
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Andere Bezeichnung
- Cytokeratin 14
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Hintergrund
- CK-14, Dowling Meara, ebs3, ebs4, Epidermolysis Bullosa Simplex, Keratin Type I Cytoskeletal 14, Koebner, NFJ,Cytokeratin 14 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P02533
Target
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