Rekombinanter Cytokeratin 5 Antikörper (AA 316-590)

Produktnummer ABIN7477433

Der Maus Monoklonal Anti-Cytokeratin 5-Antikörper wurde für IHC validiert. Er ist geeignet, Cytokeratin 5 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Cytokeratin 5 Antikörper (AA 316-590) (ABIN7477433)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 5 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-605M

Produktdetails anti-Cytokeratin 5 Antikörper

Bindungsspezifität: AA 316-590

Immunogen: Recombinant fragment (around aa 316-590) of human Cytokeratin 5 (KRT5) protein (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Tonsil: A moderate to strong cytoplasmic KRT5 staining should be seen in virtually all squamous epithelial cells. Prostate: A strong cytoplasmic staining should be present in the majority of basal cells.

Negative Control: Tonsil: Lymphatic cells must not show any KRT5 positivity.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Andere Bezeichnung: Cytokeratin 5

Hintergrund: 58 kDa Cytokeratin, CK5, Cytokeratin-5, DDD1, Epidermolysis Bullosa Simplex 2 (EBS2), Keratin 5, Keratin, Type II Cytoskeletal 5, Keratin-5, KRT5, Type-II Cytoskeletal 5, Type-II keratin Kb5,Cytokeratin 5 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P13647