CD163 Antikörper (AA 43-196)

Produktnummer ABIN7477417

Dieses Anti-CD163-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD163 in IHC. Geeignet für Human.

Kurzübersicht für CD163 Antikörper (AA 43-196) (ABIN7477417)

Target: CD163

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD163 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-163M

Produktdetails anti-CD163 Antikörper

Bindungsspezifität: AA 43-196

Immunogen: Recombinant fragment of human CD163 protein (around aa 43-196) (exact sequence is proprietary)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: A moderate to strong cytoplasmic staining of macrophages should be seen in the interfollicular zones of the tonsil, lamina propria of the appendix and in the Kupffer cells of the liver (see images). Macrophages surrounding the vessels in brain specimens should stain positive, while microglial cells should not show CD163 staining.

Negative Control: CD163 staining should not be seen in hepatocytes and in epithelial cells of the appendix and tonsil.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu CD163

Target: CD163

Andere Bezeichnung: CD163

Hintergrund: CD163, CD163 antigen, Macrophage-associated antigen, M130, CD163 Molecule, Hemoglobin scavenger receptor, MM130, Scavenger receptor cysteine rich type 1 protein M130,CD163 antibody validated for immunohistochemistry on 76 different Normal Tissues