Rekombinanter CD34 Antikörper

Produktnummer ABIN7477411

Dieser Anti-CD34 Antikörper ist ein Kaninchen Monoklonal Antikörper zur Detektion von CD34 in IHC. Geeignet für Human.

Kurzübersicht für Rekombinanter CD34 Antikörper (ABIN7477411)

Target: CD34

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MSVA-034R

Produktdetails anti-CD34 Antikörper

Immunogen: Recombinant full-length human HPCA1 protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IHC 1:100-1:200

Kommentare:

Positive Control: Liver: A moderate to strong, predominantly membranous staining of endothelial cells should be seen in portal vessels and periportal sinusoidal endothelial cells. Endothelial cells located more remotely from the portal field show a rapidly decreasing CD34 expression level.

Negative Control: Liver: Hepatocytes should always stain CD34 negative. Sinusoidal cells remote from the portal fields must also be CD34 negative.

Protokoll: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34

Hintergrund: Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin,CD34 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P28906