Rekombinanter CD8 Antikörper (AA 135-235)
Kurzübersicht für Rekombinanter CD8 Antikörper (AA 135-235) (ABIN7477399)
Target
Alle CD8 Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
-
-
Bindungsspezifität
- AA 135-235
-
Immunogen
- Synthetic peptide corresponding to CD8α residues within aa135-235 of CD8α
-
Isotyp
- IgG
-
-
-
-
Applikationshinweise
- IHC 1:100-1:200
-
Kommentare
-
Positive Control: Tonsil (interfollicular areas)
Negative Control: Epithelial tissues, majority of cells in germinal centres of lymphatic organs
-
Protokoll
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Liquid
-
Lagerung
- 4 °C,-20 °C,-80 °C
-
Informationen zur Lagerung
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- CD8
-
Andere Bezeichnung
- CD8
-
Hintergrund
- CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain,CD8 antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P01732
Target
-