Double-Strand Break Repair Protein MRE11 (MRE11) (C-Term) Antikörper

Produktnummer ABIN7468278

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch Double-Strand Break Repair Protein MRE11 in ICC, IF, IP und WB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Double-Strand Break Repair Protein MRE11 (MRE11) (C-Term) Antikörper (ABIN7468278)

Target: Double-Strand Break Repair Protein MRE11 (MRE11)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)

Güteklasse: KO Validated

Produktdetails

Bindungsspezifität: C-Term

Kreuzreaktivität: Human

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human Mre11. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: HeLa , MRE11A-transfected 293T Validation: Comparison, KO/KD, Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.57 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Antigendetails

Target: Double-Strand Break Repair Protein MRE11 (MRE11)

Andere Bezeichnung: MRE11 homolog, double strand break repair nuclease

Hintergrund:

Synonyms: MRE11 homolog, double strand break repair nuclease , ATLD , HNGS1 , MRE11A , MRE11B

Background: This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog, this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq]

Molekulargewicht: 81 kDa

Gen-ID: 4361

UniProt: P49959