ESR2 Antikörper (AA 1-153)

Produktnummer ABIN7464811

Der Maus Monoklonal Anti-ESR2-Antikörper wurde für WB, IHC, IF, FACS, ICC, IHC (p), IHC (fro), ChIP, IHC (fp) und DB validiert. Er ist geeignet, ESR2 in Proben von Human zu detektieren.

Kurzübersicht für ESR2 Antikörper (AA 1-153) (ABIN7464811)

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ESR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Dot Blot (DB)

Güteklasse: KO Validated

Klon: 14C8

Produktdetails anti-ESR2 Antikörper

Bindungsspezifität: AA 1-153

Spezifität: Strong Erb staining in epithelial cell nuclei. Occasional weak to moderate staining is seen in surrounding stromal and endothelial cell nuclei. Sporadic light cytoplasmic staining is sometimes observed. We have also successfully detected ERb in colon and ovarian tumors using the same antibody.,Knockdown/Knockout validation was supported by references (PMID:27323858)

Kreuzreaktivität: Human, Affe, Maus

Aufreinigung: Protein G purified

Immunogen: Amino acids 1-153 of human ER-beta expressed in E. coli.

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: For IHC, a recommended protocol is provided. For WB: Use at a concentration of 1-5 μg/mL. For ChIP: Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.

Kommentare:

Positive Control: Myc-DDK-tagged ESR2-transfected 293T

Validation: Comparison, KO/KD, Overexpression

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.23 mg/mL

Buffer: PBS, No Preservative

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu ESR2

Target: ESR2 (Estrogen Receptor 2 (ESR2))

Andere Bezeichnung: estrogen receptor 2

Hintergrund: Estrogen receptor 2 , ER-BETA , ESR-BETA , ESRB , ESTRB , Erb , NR3A2 , ODG8,This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq, Jul 2008]

Molekulargewicht: 59 kDa

Gen-ID: 2100

UniProt: Q92731

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling