CA9 Antikörper (N-Term)

Produktnummer ABIN7464487

Der Maus Monoklonal Anti-CA9-Antikörper wurde für WB, IF und ICC validiert. Er ist geeignet, CA9 in Proben von Human zu detektieren.

Kurzübersicht für CA9 Antikörper (N-Term) (ABIN7464487)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CA9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: GT14212

Produktdetails anti-CA9 Antikörper

Bindungsspezifität: N-Term

Kreuzreaktivität: Human

Aufreinigung: Affinity purified by Protein G.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human Carbonic Anhydrase IX. The exact sequence is proprietary.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: HeLa (200 μM CoCl2 treatment for 24 hr) , 30u g HeLa (200 μM CoCl2 treatment for 48 hr) , HeLa (1% O2 treatment for 24 hr) , HeLa (1% O2 treatment for 48 hr)

Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, No Preservative

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Andere Bezeichnung: carbonic anhydrase 9

Hintergrund: Carbonic anhydrase 9 , CAIX , MN,Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]

Molekulargewicht: 50 kDa

Gen-ID: 768

UniProt: Q16790