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Vimentin Antikörper

Dieses Anti-Vimentin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Vimentin in WB, ELISA, IF, FACS, IHC (p), ICC, IP und IHC (fro). Geeignet für Human.
Produktnummer ABIN7464236

Kurzübersicht für Vimentin Antikörper (ABIN7464236)

Target

Alle Vimentin (VIM) Antikörper anzeigen
Vimentin (VIM)

Reaktivität

  • 377
  • 131
  • 129
  • 63
  • 62
  • 50
  • 44
  • 39
  • 31
  • 23
  • 21
  • 14
  • 13
  • 8
  • 8
  • 6
  • 6
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 199
  • 164
  • 11
  • 6
  • 6
  • 5
  • 2
  • 2
Maus

Klonalität

  • 203
  • 191
Monoklonal

Konjugat

  • 241
  • 19
  • 13
  • 12
  • 9
  • 7
  • 6
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Dieser Vimentin Antikörper ist unkonjugiert

Applikation

  • 328
  • 171
  • 125
  • 106
  • 93
  • 62
  • 62
  • 40
  • 39
  • 33
  • 33
  • 32
  • 23
  • 8
  • 6
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Güteklasse

KO Validated

Klon

GT812
  • Spezifität

    IP/MS validation was supported by references (PMID:30377427)

    Kreuzreaktivität

    Cat, Human, Maus, Ratte

    Aufreinigung

    Affinity purified by Protein G.

    Immunogen

    Recombinant protein encompassing a sequence within the center region of human Vimentin. The exact sequence is proprietary.

    Isotyp

    IgG2b
  • Applikationshinweise

    WB: 1:500-1:75000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

    Kommentare

    Positive Control: 293T , HeLa , U87-MG , SK-N-SH , IMR32 , SK-N-AS , Rat-2 , Neuro2A , C8D30 , NIH-3T3 , C2C12

    Validation: Comparison, IP/MS, KO/KD, Orthogonal, Overexpression

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    PBS, No Preservative

    Konservierungsmittel

    Without preservative

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Target

    Vimentin (VIM)

    Andere Bezeichnung

    vimentin

    Hintergrund

    Vimentin,Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]

    Molekulargewicht

    54 kDa

    Gen-ID

    7431

    UniProt

    P08670

    Pathways

    Caspase Kaskade in der Apoptose
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