Rekombinanter MED1 Antikörper (AA 1550-1581)

Produktnummer ABIN7455140

Dieses Anti-MED1-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von MED1 in WB, IP, ICC, IHC (fp) und FACS. Geeignet für Human und Maus.

Kurzübersicht für Rekombinanter MED1 Antikörper (AA 1550-1581) (ABIN7455140)

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MED1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Flow Cytometry (FACS)

Klon: BLR037F

Produktdetails anti-MED1 Antikörper

Bindungsspezifität: AA 1550-1581

Verwendungszweck: Rabbit anti-MED1 Recombinant Monoclonal Antibody [BLR037F]

Homologie: Orangutan

Immunogen: between AA 1550 and 1581 (C-term)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 20 μL/1 mg lysate

ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

WB: 17:40:00

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 100 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu MED1

Target: MED1 (Mediator Complex Subunit 1 (MED1))

Andere Bezeichnung: MED1

Hintergrund: Background: MED1 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) Q15648].

Gen-ID: 5469

NCBI Accession: NP_004765

UniProt: Q15648

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha