BRCC3 Antikörper (AA 266-316)

Produktnummer ABIN7454833

Der Kaninchen Polyklonal Anti-BRCC3-Antikörper wurde für WB, IP und IHC (fp) validiert. Er ist geeignet, BRCC3 in Proben von Human und Maus zu detektieren.

Kurzübersicht für BRCC3 Antikörper (AA 266-316) (ABIN7454833)

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRCC3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-BRCC3 Antikörper

Bindungsspezifität: AA 266-316

Verwendungszweck: Rabbit anti-BRCC36 Antibody, Affinity Purified

Homologie: Orangutan

Aufreinigung: Affinity Purified

Immunogen: between AA 266 and 316

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu BRCC3

Target: BRCC3 (BRCA1/BRCA2-Containing Complex, Subunit 3 (BRCC3))

Andere Bezeichnung: BRCC36

Hintergrund: Background: BRCA1/BRCA2-containing complex subunit 36 (BRCC36) is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. BRCC36 is a component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites and leads to targeting of the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). In the BRCA1-A complex, BRCC36 specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs) [taken from the Universal Protein Resource (UniProt) P46736].

Gen-ID: 79184

NCBI Accession: NP_077308

UniProt: P46736

Pathways: Positive Regulation of Response to DNA Damage Stimulus