RNF20 Antikörper (AA 125-175)

Produktnummer ABIN7454669

Dieses Anti-RNF20-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RNF20 in WB, IP und IHC (fp). Geeignet für Human und Maus.

Kurzübersicht für RNF20 Antikörper (AA 125-175) (ABIN7454669)

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNF20 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-RNF20 Antikörper

Bindungsspezifität: AA 125-175

Verwendungszweck: Rabbit anti-RNF20 Antibody, Affinity Purified

Homologie: Chicken,Bovine

Aufreinigung: Affinity Purified

Immunogen: between AA 125 and 175

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu RNF20

Target: RNF20 (Ring Finger Protein 20 (RNF20))

Andere Bezeichnung: RNF20

Hintergrund: Background: The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes. Targeting of proteins by ubiquitination occurs in three main steps that involve the activation of ubiquitin by E1 enzymes, the carrying of ubiquitin to the target protein by E2 enzymes, and the ligation of ubiquitin to the target protein by E3 enzymes. Ring finger protein 20 (RNF20) is a member of the RING type E3 ubiquitin ligases. RNF20 forms a complex with RNF40 to carry out H2B monoubiquitination in association with hPAF and the UbcH6 ubiquitin E2-conjugating enzyme. H2B monoubiquitination by RNF40/RNF20 has been shown to be associated with increased H3 methylation and gene transcription.

Gen-ID: 56254

NCBI Accession: NP_062538

UniProt: Q5VTR2