Claspin Antikörper (AA 725-775)

Produktnummer ABIN7454603

Dieses Anti-Claspin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Claspin in WB, IP und IHC (fp). Geeignet für Human.

Kurzübersicht für Claspin Antikörper (AA 725-775) (ABIN7454603)

Target: Claspin (CLSPN)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Claspin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-Claspin Antikörper

Bindungsspezifität: AA 725-775

Verwendungszweck: Rabbit anti-Claspin Antibody, Affinity Purified

Homologie: Orangutan,Gorilla,Chimpanzee

Aufreinigung: Affinity Purified

Immunogen: between AA 725 and 775

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 1 - 5 μg/mg lysate

WB: 1:500 - 1:2,500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu Claspin

Target: Claspin (CLSPN)

Andere Bezeichnung: Claspin

Hintergrund: Background: Claspin is a checkpoint protein that interacts with and regulates checkpoint kinase 1 (Chk1) in response to replication stress. The interaction between claspin and Chk1 has been demonstrated to be required for ATR-dependent Chk1 activation. The interaction involves the phosphorylation of claspin that may be accomplished by Chk1 kinase activity. Claspin has been found to localize to chromatin in both the presence and absence of damaged DNA and to associate with PCNA, as an essential component of the DNA replication machinery. This association with the replication machinery may indicate a role for claspin in linking replication to DNA damage signaling and Chk1 activation.

Gen-ID: 63967

UniProt: Q9HAW4