MDC1 Antikörper (AA 925-975)

Produktnummer ABIN7454586

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MDC1 in WB, IP und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MDC1 Antikörper (AA 925-975) (ABIN7454586)

Target: MDC1 (Mdc1 (MDC1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MDC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-MDC1 Antikörper

Bindungsspezifität: AA 925-975

Verwendungszweck: Rabbit anti-MDC1 Antibody, Affinity Purified

Aufreinigung: Affinity Purified

Immunogen: between AA 925 and 975

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 4 μg/mg lysate

WB: 1:1,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu MDC1

Target: MDC1 (Mdc1 (MDC1))

Andere Bezeichnung: MDC1

Hintergrund: Background: Mediator of DNA damage checkpoint protein 1 (Mdc1) is required for checkpoint mediated cell cycle arrest in response to DNA damage within both the S phase and G2/M phases of the cell cycle. It may serve as a scaffold for the recruitment of DNA repair and signal transduction proteins to discrete foci of DNA damage marked by 'Ser-139' phosphorylation of histone H2AFX. It is also required for downstream events subsequent to the recruitment of these proteins. These include phosphorylation and activation of the ATM, CHEK1 and CHEK2 kinases, and stabilization of TP53 and apoptosis [taken from the Universal Protein Resource (UniProt) Q5PSV9].

Gen-ID: 9656

NCBI Accession: NP_055456

UniProt: Q14676