MED15 Antikörper (AA 450-500)

Produktnummer ABIN7453728

Dieses Anti-MED15-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MED15 in WB, IP und IHC (fp). Geeignet für Human.

Kurzübersicht für MED15 Antikörper (AA 450-500) (ABIN7453728)

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED15 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-MED15 Antikörper

Bindungsspezifität: AA 450-500

Verwendungszweck: Rabbit anti-MED15 Antibody, Affinity Purified

Homologie: Mouse

Aufreinigung: Affinity Purified

Immunogen: between AA 450 and 500

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Tris-buffered Saline containing 0.1 % BSA and 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu MED15

Target: MED15 (Mediator Complex Subunit 15 (MED15))

Andere Bezeichnung: MED15

Hintergrund: Background: Mediator complex subunit 15 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. MED15 is required for cholesterol-dependent gene regulation. It also positively regulates the Nodal signaling pathway. [taken from the Universal Protein Resource (UniProt) Q96RN5].

Gen-ID: 51586

NCBI Accession: NP_001003891

UniProt: Q96RN5

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha