MED12 Antikörper (C-Term)

Produktnummer ABIN7453548

Der Kaninchen Polyklonal anti-MED12 Antikörper (ABIN7453548) detektiert spezifisch MED12 in WB, IP und IHC (fp). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für MED12 Antikörper (C-Term) (ABIN7453548)

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MED12 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-MED12 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Rabbit anti-MED12 Antibody, Affinity Purified

Homologie: Chimpanzee

Aufreinigung: Affinity Purified

Immunogen: between AA 2127 and C-term

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:2,000 - 1:10,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu MED12

Target: MED12 (Mediator Complex Subunit 12 (MED12))

Andere Bezeichnung: MED12

Hintergrund: Background: MED12 is a component of the multisubunit mediator complex that functions as an interface between gene-specific transcription factors and the RNA Pol II/general transcription factor machinery. The mediator complex was first identified as a coactivator of the thyroid hormone receptor and has since been observed to function as a coactivator of a variety of transcription factors. In this context, MED12 interaction with beta-catenin has been shown to be required for Wnt-signaling dependent transcription.

Gen-ID: 9968

NCBI Accession: NP_005111

UniProt: Q93074

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Tube Formation