NUCKS1 Antikörper (AA 147-197)

Produktnummer ABIN7453492

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch NUCKS1 in WB, IP und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für NUCKS1 Antikörper (AA 147-197) (ABIN7453492)

Target: NUCKS1 (Nuclear Casein Kinase and Cyclin-Dependent Kinase Substrate 1 (NUCKS1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NUCKS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Produktdetails anti-NUCKS1 Antikörper

Bindungsspezifität: AA 147-197

Verwendungszweck: Rabbit anti-NUCKS Antibody, Affinity Purified

Homologie: Bovine

Aufreinigung: Affinity Purified

Immunogen: between AA 147 and 197

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 5 μg/mg lysate

WB: Not recommended. ABIN7453492 has not performed satisfactorily when used for WB of NUCKS in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of NUCKS.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu NUCKS1

Target: NUCKS1 (Nuclear Casein Kinase and Cyclin-Dependent Kinase Substrate 1 (NUCKS1))

Andere Bezeichnung: NUCKS

Hintergrund: Background: NUCKS (nuclear ubiquitous casein and cyclin-dependent kinases substrate) is a nuclear phosphoprotein phosphorylated by Cdk1 during mitosis. It is a DNA-binding protein and bears two putative nuclear localization signals. NUCKS has been shown to localize to the cytoplasm in mitotic cells and is targeted to the nucleus during telophase. It is expressed ubiquitously and possesses features of a housekeeping gene.

Gen-ID: 64710

NCBI Accession: NP_073568

UniProt: Q9H1E3