hPrp3p (AA 100-150) Antikörper

Produktnummer ABIN7453461

Der Kaninchen Polyklonal anti-hPrp3p Antikörper (ABIN7453461) detektiert spezifisch hPrp3p in IP, IHC (fp) und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Maus.

Kurzübersicht für hPrp3p (AA 100-150) Antikörper (ABIN7453461)

Target: hPrp3p

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)

Produktdetails

Bindungsspezifität: AA 100-150

Verwendungszweck: Rabbit anti-hPrp3p Antibody, Affinity Purified

Homologie: Chicken,Bovine,Orangutan

Aufreinigung: Affinity Purified

Immunogen: between AA 100 and 150

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: Not recommended. ABIN7453461 has not performed satisfactorily when used for WB of hPrp3p in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of hPrp3p.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Antigendetails

Target: hPrp3p

Hintergrund: Background: hPrp3p (human pre-mRNA splicing factor 3) was identified as a factor that associated with U4/U6 snRNPs which are components of the splicesome. hPrp3p is homologous to S. cerevisiae splicing factor Prp3p. Mutations in the PRPF3 gene are associated with retinitis pigmentosa-18. It has been determined that mutations in hPrp3p may alter the recognition motif for phosphorylation by casein kinase II (CKII) and that loss of phosphorylation may result in aberrant interactions within the U4/U6 snRNP complex.

Gen-ID: 9129

NCBI Accession: NP_004689

UniProt: O43395