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HSP90 alpha/beta Antikörper

Dieses Anti-HSP90 alpha/beta-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von HSP90 alpha/beta in WB, IF, IHC (p) und IP. Geeignet für Human, Ratte und Maus.
Produktnummer ABIN7384453

Kurzübersicht für HSP90 alpha/beta Antikörper (ABIN7384453)

Target

HSP90 alpha/beta (Heat Shock Protein 90 alpha/beta (HSP90 alpha/beta))

Reaktivität

  • 29
  • 22
  • 20
  • 6
  • 5
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Ratte, Maus

Wirt

  • 17
  • 10
  • 2
Kaninchen

Klonalität

  • 16
  • 12
Monoklonal

Konjugat

  • 10
  • 3
  • 3
  • 3
  • 3
  • 3
  • 1
  • 1
  • 1
  • 1
Dieser HSP90 alpha/beta Antikörper ist unkonjugiert

Applikation

  • 28
  • 25
  • 14
  • 7
  • 6
  • 5
  • 2
  • 2
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Klon

R08-8A1
  • Aufreinigung

    Affinity Purified

    Isotyp

    IgG
  • Applikationshinweise

    WB 1:1000-1:2000 IHC 1:50-1:200 IF 1:50-1:200

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    300 μg/mL

    Buffer

    50 mM Tris-Glycine( pH 7.4), 0.15M NaCl, 40 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    -20 °C

    Informationen zur Lagerung

    Store at -20°C. Avoid freeze / thaw cycles.
  • Target

    HSP90 alpha/beta (Heat Shock Protein 90 alpha/beta (HSP90 alpha/beta))

    Andere Bezeichnung

    Hsp90 alpha/beta

    Hintergrund

    HSP84,HSPC2,HSPCB,D6S182,HSP90B,Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function (PubMed:16478993, PubMed:19696785). Engages with a range of client protein classes via its interaction with various co-chaperone proteins or complexes, that act as adapters, simultaneously able to interact with the specific client and the central chaperone itself. Recruitment of ATP and co-chaperone followed by client protein forms a functional chaperone. After the completion of the chaperoning process, properly folded client protein and co-chaperone leave HSP90 in an ADP-bound partially open conformation and finally, ADP is released from HSP90 which acquires an open conformation for the next cycle (PubMed:27295069, PubMed:26991466). Apart from its chaperone activity, it also plays a role in the regulation of the transcription machinery. HSP90 and its co-chaperones modulate transcription at least at three different levels. In the first place, they alter the steady-state levels of certain transcription factors in response to various physiological cues. Second, they modulate the activity of certain epigenetic modifiers, such as histone deacetylases or DNA methyl transferases, and thereby respond to the change in the environment. Third, they participate in the eviction of histones from the promoter region of certain genes and thereby turn on gene expression (PubMed:25973397). Antagonizes STUB1-mediated inhibition of TGF-beta signaling via inhibition of STUB1-mediated SMAD3 ubiquitination and degradation (PubMed:24613385). Promotes cell differentiation by chaperoning BIRC2 and thereby protecting from auto-ubiquitination and degradation by the proteasomal machinery (PubMed:18239673). Main chaperone that is involved in the phosphorylation/activation of the STAT1 by chaperoning both JAK2 and PRKCE under heat shock and in turn, activates its own transcription (PubMed:20353823).

    Molekulargewicht

    Observed_MW: 90kDa

    Calculated_MW: 85/83kDa

    Gen-ID

    3326
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