BEBOV GP Antikörper

Produktnummer ABIN7383891

Der Kaninchen Polyklonal Anti-BEBOV GP-Antikörper wurde für WB validiert. Er ist geeignet, BEBOV GP in Proben von Ebola Virus zu detektieren.

Kurzübersicht für BEBOV GP Antikörper (ABIN7383891)

Target: BEBOV GP (Bundibugyo Ebola Virus Envelope Glycoprotein (BEBOV GP))

Reaktivität: Ebola Virus

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB)

Produktdetails anti-BEBOV GP Antikörper

Spezifität: Anti-Ebola virus EBOV(subtype Bundibugyo, strain Uganda 2007) GP1/Glycoprotein Polyclonal Antibody

Aufreinigung: Antigen Affinity

Immunogen: Recombinant EBOV (subtype Bundibugyo, strain Uganda 2007) GP1 / Glycoprotein Protein (His Tag), ABIN7198902

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-1:5000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 0.2 μm filtered solution in PBS

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu BEBOV GP

Target: BEBOV GP (Bundibugyo Ebola Virus Envelope Glycoprotein (BEBOV GP))

Andere Bezeichnung: BEBOV Glycoprotein/GP1

Hintergrund: Glycoprotein,GP,The fourth gene of the EBOV genome encodes a 16- kDa envelope-attached glycoprotein (GP) and a 11 kDa secreted glycoprotein (sGP). Both GP and sGP have an identical 295-residue N-terminus, however, they have different C-terminal sequences. Recently, great attention has been paid to GP for vaccines design and entry inhibitors isolation. GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . The GP1 subunit contains a mucin domain and a receptor-binding domain (RBD), the GP2 subunit has a fusion peptide, a helical heptad-repeat (HR) region, a transmembrane (TM) domain, and a 4-residue cytoplasmic tail. The RBD of GP1 mediates the interaction of EBOV with cellular receptor (e.g. DC-SIGN/LSIGN, TIM-1, hMGL, NPC1, β-integrins, folate receptor-α, and Tyro3 family receptors), of which TIM1 and NPC1 are essential for EBOV entry, the mucin domain having N- and O-linked glycans enhances the viral attachment to cellular hMGL, and participates in shielding key neutralization epitopes, which helps the virus evades immune elimination. There are large conformation changes of GP2 during membrane fusion, which enhance the insertion of fusion loop into cellular membrane and facilitate the release of viral nucleocapsid core to cytoplasm.