tdTomato Antikörper (DyLight 550)

Produktnummer ABIN7273113

Der Ziege Polyklonal Anti-tdTomato-Antikörper wurde für IF, IHC (fro) und WB validiert. Er ist geeignet, tdTomato in Proben von Discosoma zu detektieren.

Kurzübersicht für tdTomato Antikörper (DyLight 550) (ABIN7273113)

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Reaktivität: Discosoma

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser tdTomato Antikörper ist konjugiert mit DyLight 550

Applikation: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)

Produktdetails anti-tdTomato Antikörper (DyLight 550)

Kreuzreaktivität (Details): Reacts against tdTomato, mCherry, red fluorescent protein (DsRed) from so-called disc corals of Discosoma species.

Aufreinigung: Epitope affinity purified

Immunogen: Purified recombinant peptide produced in E. coli

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB:1:500-1:2,000, IF:1:50-1:500, IHC-F:1:50-1:1,000

Kommentare:

In 293HEK cells transfected with cds plasmid detects a band of 55 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent protein).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 2.5 mg/mL

Buffer: PBS, 20 % glycerol and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20 °C for long-term storage. Store at 2-8 °C for up to one month.

Details zu tdTomato

Target: tdTomato (tdTomato Fluorescent Protein (tdTomato))

Andere Bezeichnung: tdTomato

Hintergrund: TdTomato protein is derived from DsRed, an engineered red fluorescent protein from so-called disc corals of the genus Discosoma. It is a genetic fusion of two copies of the dTomato gene, which has been specifically designed for low aggregation. It´s brightness and emission wavelength, makes it ideal for live animal research.